Own eggs and
partner’s sperm

This treatment method is recommended when natural pregnancy cannot occur naturally and when infertility has been diagnosed. During the natural menstrual cycle each month only one egg develops. For in vitro insemination it is advisable to obtain more than one ovum simultaneously. Therefore hormone stimulates are used to obtain several mature eggs simultaneously. This treatment program is recommended when the amount of spermatozoids is not sufficient for artificial insemination or when fallopian tubes are not passable or are damaged. This method is also prescribed after unsuccessful attempts of natural pregnancy when infertility has been diagnosed.

1. Stimulation of ovaries.

Doctor-reproductologist will draw a customized program of hormonal stimulation of ovaries using FSH – hormones that stimulate follicles. As a result more eggs are developed than during a natural cycle.

The process takes ten to twelve days. Using ultrasonic testing your doctor carefully monitors the growth of follicles. If necessary, treatment program can be adjusted to increase its effectiveness.

2. Retrieving of ovum

The ovum collection procedure is carried out under anesthesia. Under ultrasound guiding follicular liquid is sucked from ovaries using a thin needle. The procedure takes no more than ten to twenty minutes.

3. Fertilization

The main factor determining the treatment method is quality of sperm. Amount and motility of spermatozoids influence the choice of treatment. Ovum can be fertilized using two basic methods:
1. IVF — spermatozoids and ovum are placed together, and the next day amount of fertilized eggs is counted.
2. Micro Insemination by intra cytoplasmic sperm injection ICSI – spermatozoids are injected directly into cytoplasm of the eggs.

Most active spermatozoids are separated from the rest, and insemination is carried out a couple of hours after retrieval of ovum.

4. Selection of embryo

The ovum is inseminated in the incubator, where a particular temperature and level of carbon dioxide are maintained. When the ovum begins to divide, it becomes an embryo. Embryos are classified according to the number of cells and quality of their structure. The best are chosen to be transferred to the uterus, the remaining embryos are frozen. Sometimes cultivation of embryos in the laboratory is continued for five days. It is called cultivation of embryos to the blastocyst stage.

In some cases that helps to select the most viable embryos. The decision about cultivation of embryos to the blastocyst stage is made by a doctor and embryologist.

5. Transferring embryo to the uterus

In most cases embryos are transferred to the uterus on the third day after ovarian puncture. Usually one or two embryos are transferred.

Transfer of one embryo is recommended when it has a high quality, patient is in reproductive age, and it is her first attempt. Chances of pregnancy can be increased by a transfer of two embryos, however, that might increase the possibility of twin pregnancy.

The transfer procedure is always fast and painless. Embryos are transferred through vagina into a womb using a thin plastic catheter. Pregnancy begins if the embryo has attached itself to the lining of uterus wall several days after the transfer to the uterine cavity.

6. Period from the transfer of embryo until pregnancy test

A reliable pregnancy test can be carried out two weeks after implantation. For many this fortnight of waiting is the most difficult period of the treatment.

All one can do is wait and hope that the embryo will attach itself to the lining of uterus wall, and pregnancy will begin.

During this period patient's mood swings from optimism and euphoria to deep despair. Your doctor, nurses and other experts of AVA CLINIC will help you, provide support and inspiration.

7. Pregnancy test

Blood pregnancy test is carried out twelve days after the embryo transfer.

Urine pregnancy test is carried out fourteen days after embryo transfer.

The team of AVA CLINIC is as excited about finding out test results as you are. We kindly ask you to let us know the results as soon as possible.

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